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. 2018 Aug 20;8:12422. doi: 10.1038/s41598-018-30853-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Plasmid maps of pKaKa3, pKaKa4, and pKaKa5 to expand the utility of λ-red recombineering to non-naturally competent Burkholderia species. Abbreviations: araC on pKaKa3 and pKaKa5, activator of the arabinose-inducible promoter (P_ara) from E. coli; araBCDEFGHI on pKaKa4, B. thailandensis arabinose utilization operon³⁸; gam-exo-bet, λ-red recombineering genes³⁹; mob; RP4-dependent conjugal origin of transfer of B. bronchiseptica cryptic plasmid pBBR1; ori-rep; bhr replicon of B. bronchiseptica pBBR1 plasmid⁴⁰; nptII, encodes kanamycin resistance⁴¹; P_ara, arabinose inducible promoter⁴²; P_rha, rhamnose inducible promoter⁴³; PC_S12, constitutive promoters of B. pseudomallei and B. cenocepacia rpsL gene⁴⁴; pheS, engineered gene encoding a mutant version of α-subunit of phenylalanyl tRNA synthase⁴⁵; rhaR and rhaS, regulators of the rhamnose inducible promoter⁴³; sacB, encoding for a modified levansucrase counter-selectable marker⁴⁶. Tc^R, tetracycline resistance.