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. 2018 Aug 20;8:12422. doi: 10.1038/s41598-018-30853-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo attenuation of Bm Δasd strains in BALB/c intranasal challenge. BALB/c mice (n = 5) were challenged intranasally with 1 × 10⁷ CFU of Bm strains ATCC23344 (a), Ivan (b), China 5 (c), 2002721278 (d) and their Δasd counterparts. Survival was monitored for 63 days (left panels). Surviving mice were sacrificed and bacterial burdens from the lungs, liver, and spleen were determined by serial dilution and plating (right panels).