Fig. 2.

Multistep surface chemistry to functionalise ITO surfaces to control average RGD spacing. a Schematic of the formation of the interface where the surface density of RGD peptides attached to the otherwise cell-inert surface (facilitated by hexa(ethylene oxide) molecules, EO₆) was controlled by controlling the ratio of the molar ratio of GRGDC-Alexa Fluor 647 (RGD) and unlabelled, nonfunctional GRGE (RGE) peptides. b RGD peptides imaged by dSTORM showing the number of RGD per μm² on functionalised ITO surfaces. RGD density was determined from SMLM data of n = 4 independent experiments. Mean spacing was calculated from RGD densities, assuming a spatially random distribution