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. 2018 Aug 21;4:52. doi: 10.1038/s41421-018-0041-2

Fig. 2. UCP1 expressions in human pericardial adipose tissue from patients with vs. without CAD.

Fig. 2

a Representative HE microscopic picture and immunohistochemical staining of UCP1 of epicardial and paracardial adipose tissue samples from patient with or without CAD. b Quantification of UCP-1+ area of EAT and PAT from patients with or without CAD. (Data were collected using Image Pro-Plus software for IHC analysis of three individual patients, three fields per person.) c, d Western blot experiment using antibodies against UCP1, PGC1α, Cidea, and Actin of EAT and PAT from patients with or without CAD. e, f Real-time PCR data showing the fold induction of indicated brown adipocyte-related genes with expression normalized to the housekeeping gene 18s in EAT and PAT from patients with or without CAD (n = 12–16). g, h Real-time PCR data showing the fold induction of UCP1 with expression normalized to the housekeeping gene 18s in EAT and PAT from patients with different level of CAD (n = 8–13)