Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 21;4:52. doi: 10.1038/s41421-018-0041-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 8 — On day 4 after adipogenic induction, RPS3A was silenced by siRPS3A. The indicated cells were then harvested on day 8 post induction and subjected to further studies. a Confocal microscopic images of mature brown adipocytes immunostained for mitochondria (red) and DAPI (blue). b Values are the percentage of mitochondria⁺ to DAPI⁺ cells (n = 3). c Western blotting was conducted to measure the level of the indicated proteins. d Mature brown adipocytes were treated with pAd-lacZ/pAd-RPS3A on day 4 after adipogenic induction and collected for Western blotting to determine the indicated proteins. e Confocal microscopic images of brown preadipocytes at different time points during differentiation immunostained for mitochondria (red), RPS3A (green), and DAPI (blue). f Cells were separated into cytosol and mitochondrial fractions, tubulin was used as marker for the cytosol, cytochrome C was used as a mitochondrial marker. g Schematic model illustrates the role of pericardial adipose tissue in atherosclerosis and CAD. The browning of pericardial adipose tissue (both EAT and PAT) plays an important role in maintaining the function of vascular and blood flow. The decreasing expression of RPS3A promotes the disorder of mitochondria and metabolism from periaortic adipose tissue (EAT), which contributes to atherosclerosis and CAD