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. 2018 Aug 20;8:12434. doi: 10.1038/s41598-018-30603-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometric analysis of HEK293 (A–C) cells expressing EGFP-fusion variants of Bax, Bak and Bok (representative western blot inlay; identical membrane was incubated with anti-GFP and anti-β-actin antibody sequentially) show induction of cell death (SytoxRed positive cells). Expression of EGFP-Bax, -Bak and -Bok in HEK293T cells induces (B) exposure of phosphatidylserine (PS) detected by Annexin V⁺-binding and (C) loss of mitochondrial membrane potential (TMRE^low) at different time points. (D) EGFP-Bax, -Bak or -Bok expression induces cell death of Bax&Bak deficient BMK cells. Cell death induction in Bax&Bak deficient BMK cells by EGFP-tagged Bax-, Bak and Bok is associated with (E) exposure of PS and (F) loss of mitochondrial membrane potential at different time points.