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. 2001 Jan;125(1):430–436. doi: 10.1104/pp.125.1.430

Figure 3.

Figure 3

Expression of neo transgenes in the plastid genome. A, Immunoblot analysis to detect NPTII. Amount of total soluble leaf protein (micrograms) loaded on the SDS-PAGE gel is indicated above the lanes. A commercial NPTII dilution series was loaded for reference. Lanes for plant lines are designated with transforming plasmid; Wt, Wild-type tobacco sample. B, NPTII detected by Coomassie Brilliant Blue R250 in SDS-PAGE gel. Twenty micrograms total soluble protein was loaded per lane. Commercially available NPTII (400 ng) was also loaded. Marked are the Rubisco large (LSU) and small (SSU) subunits. C, The levels of neo mRNA in the transplastomic leaves. Blots were probed for neo (top) and cytoplasmic 25S rRNA as loading control (bottom). D, Levels of NPTII and neo mRNA based on three to six experiments. Highest value was taken as 100%. Plant line, plasmid name (for example pHK30) and number and letter combination; + or −, SD is present or absent at prokaryotic consensus position; wt or m, downstream sequence is wild type or mutant.