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. 2018 Aug 14;9:1905. doi: 10.3389/fimmu.2018.01905

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Copyright © 2018 Kwon, Lim, Jang, Lee, Park, Kim, Yun, Shin, Jo, Oh, Roh and Choi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Modulatory effect of WIKIM30 on regulatory T cell (Treg)-related responses in mesenteric lymph nodes (MLNs) of atopic dermatitis (AD) mice. (A) Proportion of Tregs in MLNs. Isolated MLN cells were stained with antibodies against CD3, CD4, CD25, and Foxp3 and then analyzed by flow cytometry. (B) MLN cells were treated with anti-CD3/CD28 monoclonal antibodies for 48 h, and interleukin (IL)-4, IL-10, and IFN-γ levels in the culture supernatant were measured with the cytometric bead array kit. Data represent the mean ± SE of n = 5 mice per group from three independent experiments. Student’s t-test (unpaired); **p < 0.01 vs. AD group. ns, p > 0.05 vs. AD group.