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. 2018 Aug 14;9:1866. doi: 10.3389/fimmu.2018.01866

Figure 1.

Figure 1

Autophagy is increased in uremic media calcification. DBA/2 mice were fed with high-phosphate diet (HPD; gray bars) or standard chow diet (SCD; black bars) for 5 or 12 days, respectively (SCD: n = 11; HPD 5d: n = 6, HPD 12d n = 4). (A) Calcium content in kidneys was measured quantitatively. (B) Blood urea nitrogen (BUN) levels were evaluated. (C) qPCR analysis of respective autophagy related genes and Il-1beta was performed from aortas. Western Blot analysis for (D) light chain 3 (LC3) and (E) p62 levels was performed. Three independent experiments have been performed. (F) A representative western blot is provided. (G) Aortas of SCD- and HPD-fed mice were stained with Alizarin Red, with an antibody detecting LC3 or nitro blue tetrazolium (NBT). Representative pictures from aorta sections are shown. All data are presented as mean ± SEM. *p < 0.05, **p < 0.01.