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Human papillomavirus immunohistochemistry. (A,D) Hematoxylin and eosin stained sections of 2 different areas of lesion V10 (strongly PCR positive for beta-PV types 8, 12, 76, 80, and alpha-PV 57); (B,E) double staining immunofluorescence with antibodies to the E4 protein of HPV8 (green) and HPV2/57 (red); (C,F) H&E stained sections overlaid with the images from immunofluorescence microscopy to identify cells with productive HPV infection.
