Table 5.

Summary of long-term Circulating Tumor Cell culture in Lung cancer.

Study	Histology	Method of CTC isolation	CTC culture conditions	Group size	CTC lines established	Morphology/Histology	References
Hodgkinson et al., 2014	SCLC	RosetteSep/ Ficoll/ xenotransplantation	Xenotransplantation	6		Morphology of CDX macrometastases: 1. Clusters 2. Sheets of densely packed small round or oval cells 3. Scant cytoplasm 4. Enlarged/inconspicuous nuclei 5. Speckled chromatin 6. Focal nuclear molding	(142)
Hamilton et al., 2015	SCLC	Ficoll-Hypaque	RPMI 1640 medium, serum-free (insulin, IGF-1, selenite)	30	3	Spheroids or attached	(143)
Morrow et al., 2016	NSCLC	RosetteSep/Ficoll/ xenotransplantation	Xenotransplantation	1		Morphology of CDX macrometastases: 1. Diffuse sheets of large polygonal cells 2. Abundant eosinophilic cytoplasm 3. Vesicular chromatin 4. Enlarged nucleoli	(111)
Drapkin et al., 2018	SCLC	RosetteSep/Ficoll/ xenotransplantation	Xenotransplantation	46		Cytoplasmic expressions of chromogranin, synaptophysin and/or CD56 as well as the lack of CD45 expression confirmed diagnosis	(144)