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. 2018 Aug 14;9:1193. doi: 10.3389/fpls.2018.01193

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Copyright © 2018 Sepsi, Fábián, Jäger, Heslop-Harrison and Schwarzacher.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Axis distances during early meiotic prophase I in hexaploid wheat. (A) Leptotene and (B) zygotene images are single stack enlargements from samples presented in Figures 3A,B. Panel (C) is from a pachytene nucleus. Unsynapsed chromosome axes (later lateral elements) are shown as white threads (guinea pig anti-ASY1 antibody, detected by Alexa 647), the central element protein of the synaptonemal complex (rat anti-ZYP1 antibody, is pseudocoloured in purple and active centromeres (rabbit anti-CENH3 antibody) are displayed in red. Axis distances are indicated by yellow bars on (A) and (B) and purple arrows highlight closely aligned, coalescing chromosome axes. Pachytene double structures (C) seen with the anti-ZYP1 antibody are highlighted by yellow bars; a region of interest is enlarged and inserted to the upper right of the figure). Bar = 5 μm.