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. 2018 Aug 14;9:1193. doi: 10.3389/fpls.2018.01193

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Copyright © 2018 Sepsi, Fábián, Jäger, Heslop-Harrison and Schwarzacher.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Three-dimensional rendering of 2D confocal images collected from sequential focal planes of an early zygotene nucleus. Different channels represent signals from anti-CENH3 (red), anti-ASY1 (white), and anti-ZYP1 (purple) antibodies. The 3D image is turned to be observable from different angles. (A) Centromeric pole, (B) side view of the centromeric pole, (C) telomeric pole with the telomere bouquet, (D) projection turned 180° compared to the centromeric pole. (E) Optical sections collected by confocal laser scanning microscopy showing sequential focal planes of (A–D). ΔZ = 200 nm. Bar = 5 μm.