Figure 3.

Z. tritici Δku70 and ΔztstuA growth in PDB at 20°C on a rotary shaker at 200 rpm, 7 and 10 days post-inoculation (d.p.i.). Images are representative of the three independent ΔztstuA strains tested. Scale bar = 200 μm.; (A) Δku70 liquid cultures at 10 d.p.i. are black and melanised (B) ΔztstuA liquid cultures at 10 d.p.i. remain pale yellow in color with no melanisation; (C) Δku70 cultures at 7 d.p.i. stained with lactophenol cotton blue are yeast-like with some branching hyphal cells; (D) ΔztstuA cultures at 7.d.p.i. stained with lactophenol cotton blue have minimal yeast-like growth and instead produce long thread-like hyphae.