Figure 4.

Zymoseptoria tritici Δku70 and ΔztstuA growth on YPDA agar, CDV8, and PDA at 20°C, under white light (16:8 light:dark cycles), at 7 days post-inoculation (d.p.i.) and 10 d.p.i. Scale bar = 5 mm; (A) at 7 d.p.i. the Δku70 strain grows as rosy-pink yeast-like cells with some melanisation on CDV8 and PDA media. The ΔztstuA mutant grows as a white hyphal colony with no signs of yeast-like growth or melanisation; (B) at 10 d.p.i. the Δku70 strain is melanised on all three types of media, but the ΔztstuA strain remains white or rosy-pink in color with aerial hyphae.