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. 2018 Aug 14;9:1859. doi: 10.3389/fmicb.2018.01859

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Copyright © 2018 Tiley, Foster and Bailey.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Images of pycnidia (indicated by arrows) and pycnidiospores from wheat leaves infected with 4 × 10⁶ spores/ml from IPO323 and ΔztabaA, ΔztbrlA1, ΔztbrlA2, ΔztflbB, and ΔztflbC mutants. (A,G) IPO323; (B,H) ΔztabaA; (C,I) Δztbrla1; (D,J) ΔztbrlA2; (E,K) ΔztflbB; (F,L) ΔztflbC at 28 days post-infection (d.p.i.). The knock-out mutant strains were all able to produce pycnidia and pycnidiospores, and these were morphologically similar to the IPO323 strain. The ΔztbrlA2 mutant produced fewer pycnidia, and these took longer to develop compared to the IPO323 strain. Fewer spores were harvested from the ΔztbrlA2 and ΔztflbC mutants.