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. 2018 Aug 14;9:1859. doi: 10.3389/fmicb.2018.01859

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Copyright © 2018 Tiley, Foster and Bailey.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Average numbers of pycnidia and pycnidiospores produced in planta by IPO323 and the various mutant strains, assessed at 28 days post-infection (d.p.i.). Three independent knock-out strains were tested for each ΔztabaA, ΔztbrlA1, ΔztbrlA2, ΔztflbB, and ΔztflbC mutant. Bars represent mean SE across 5–10 technical repeats from a single experiment. Bars labeled with an asterisk (*) are knock-out strains with a significant difference in average pycnidia/mm² compared to IPO323 (p < 0.05). The ΔztbrlA2 strains have significantly reduced numbers of pycnidia/mm² compared to IPO323, however, there is no consistent significant difference between pycnidia/mm² produced by the ΔztabaA, ΔztbrlA1, ΔztflbB or ΔztflbC strains and those made by IPO323. The ΔztbrlA2 and ΔztflbC strains have significantly reduced numbers of pycnidiospores/ml compared to IPO323.