FIGURE 4.

MAO-B inhibition decreases ROS accumulation in response to oxidative stress in primary cultured myoblasts and myotubes from DMD patients. Primary myoblasts (A) or myotubes (B) from one healthy donor (HD 1) and DMD patients (DMD 1 and DMD 2) were loaded with Mitotracker Red CM-H₂XRos (MTR, 25 nM). Oxidative stress was induced by H₂O₂ addition (100 μM) with or without 1 μM safinamide, as a 20-min pre-treatment. Data expressed as the MTR fluorescence after 1 h from H₂O₂ were normalized to the values obtained in the absence of stimuli for each sample. Data are the mean ± SEM of three experiments per cell prep. ^∗p < 0.05.