Table 1.
Validation parameters of all trans MK-7 in oil dietary supplements.
| DAD Detector | QTOF Detector (SIM, m/z = 666.5 ± 0.5 m/z) | |
|---|---|---|
| LOD (ng mL−1) | 290.3 | 200.9 |
| % CV | 9.1 | 9.8 |
| LOQ (ng mL−1) | 957.9 | 603.7 |
| % CV | 0.3 | 9.7 |
| Recovery: | ||
| Spiked level 1: 10 µg mL−1 (%) | 85.6 | 81.4 |
| SD | 1.7 | 6.1 |
| Spiked level 2: 20 µg mL−1 (%) | 89.3 | 90.5 |
| SD | 2.3 | 6.2 |
| Spiked level 3: 30 µg mL−1 (%) | 93.8 | 94.6 |
| SD | 0.9 | 4.7 |
| Matrix effect (ME) (%) | <5 | <5 |
| SD | - | - |
| Linear (range in µg mL−1 and R2) | 0.9–250 µg mL−1 R2 = 0.997 | Very low, for example: 2–4 µg mL−1 R2 = 0.991, but for 2–20 µg mL−1 R2 = 0.860 |
| Precision and accuracy: | ||
| Intra-day precision in % CV | 0.8 | 6.1 |
| Intra-day accuracy | 2.9 | 5.8 |
| Inter-day precision in % CV | 4.1 | 11.3 |
| Inter-day accuracy | −2.9 | −5.0 |