Fig. 3.

rhIL-35 significantly decreased mRNA expression of RANK in monocytes. Effect of rhIL-35 on CD14-positive monocytes. Isolated human CD14-positive monocytes from four different donors were cultured with M-CSF (100 ng/ml) for three days. rhIL-35 (100 ng/ml) was then added for 24 hours. As a control, CD14-positive monocytes were cultured alone for 24 hours. The mRNA expression of various proteins was evaluated by RT-PCR. Percentages of mRNA expression are shown. In each panel, the right bar represents monocytes treated with rhIL-35, and the left bar represents monocytes alone (*p < 0.05). The SEM of the left bar (without rhIL-35) is zero because we normalised the values of the monocytes treated with rhIL-35 to monocytes alone