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. 2018 Jul 16;18(3):2571–2580. doi: 10.3892/mmr.2018.9287

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effect of miR-200b-3p on the viability of CRC cells. (A) Cell viability detection by Cell Counting Kit-8 assay. (B) qPCR for the expression of miR-200b-3p following transfection. (C) qPCR for the expression of mRNA of Wnt1 following transfection. (D) Western blot analysis of the expression of Wnt1 following transfection. β-actin was used as a loading control. (E) qPCR for the expression of Wnt1 following transfection. *P<0.05, **P<0.01, ***P<0.001 vs. mock group. CRC, colorectal cancer; qPCR, quantitative polymerase chain reaction; siRNA-NC, small interfering RNA negative control; si-Wnt1, small interfering RNA-Wnt1; miR, microRNA.