Skip to main content
. 2018 Jul 5;18(3):2661–2668. doi: 10.3892/mmr.2018.9252

Figure 2.

Figure 2.

HOXA10 is a candidate target of miR-665. (A) Computational analysis identified that HOXA10 may be a potential target of miR-665 and the binding sequences of HOXA10 3′-UTR and miR-665 were marked. (B) Dual luciferase assay performed in 293T cells suggested that HOXA10 was the target gene of miR-665. A significant decrease in relative luciferase activity was noted when pGL3-HOXA10-3′-UTR was cotransfected with miR-665 mimic, but not with the miR mimic NC. (C) Measurement of HOXA10 expression levels by western blotting analysis. Protein was extracted from HO8910 cells transfected with the miR-665 mimic or NC mimic. The endogenous expression levels of the β-actin protein were used for normalization and the relative HOXA10 protein expression levels are illustrated. (D) Protein was extracted from OVCAR-3 cells transfected with the miR-665 mimic or mimic NC. The endogenous expression levels of the β-actin protein were used for normalization and the relative HOXA10 protein expression levels were demonstrated. All experiments were performed in triplicate. **P<0.01. HOXA10, homeobox A10; UTR, untranslated region; miR, microRNA; NC, negative control.