Table 1.
Samples used in this study and a summary of karyotypic characteristics including the FISH signals on the chromosome 7, the lengths of chromosomes 1 and 22, the results of PCR using primer sets BP and HSA, as well as the identification results based on each approach
| Nick Name | House | FISH (signal on chr. 7) (pairs) |
Chr. 1 | Chr. 22 | Karyotype | BP | HSA | Cyt b genes | Final identification |
|---|---|---|---|---|---|---|---|---|---|
| Su-Su | Nanning Zoo | 2 | Long (Normal) |
Short (Normal) |
N. leucogenys | N/A | N/A | N/A | N/A |
| Bei-Li | Nanning Zoo | 2 | Long (Normal) |
Short (Normal) |
N. leucogenys | + | − | N. leucogenys | N. leucogenys |
| Gou-Dan | Nanning Zoo | 2 | Long (Normal) |
Short (Normal) |
N. leucogenys | + | − | N. leucogenys | N. leucogenys |
| San-Mei | Nanning Zoo | 2 | Long (Normal) |
Short (Normal) |
N. leucogenys | N/A | N/A | N/A | N/A |
| No14 | Missing | 2 | Long (Normal) |
Short (Normal) |
N. leucogenys | + | − | N. cf. leucogenys | N. cf. leucogenys |
| No name | Kunming Zoo | 2 | Long (Normal) |
Short (Normal) |
N. leucogenys | N/A | N/A | N/A | N/A |
| NB1 | Ningbo Zoo | 2 | Long (Normal) |
Short (Normal) |
N. leucogenys | N/A | N/A | N/A | N/A |
| NB2 | Ningbo Zoo | 2 | Long (Normal) |
Short (Normal) |
N. leucogenys | N/A | N/A | N/A | N/A |
| HLS3 | Huanglianshan Nature Reserve |
2 | Long (Normal) |
Short (Normal) |
N. leucogenys | N/A | N/A | N/A | N/A |
| HLS4 | Huanglianshan Nature Reserve |
2 | Long (Normal) |
Short (Normal) |
N. leucogenys | N/A | N/A | N/A | N/A |
| Fang-Fang | Nanning Zoo | 2 | Short | Long | N. siki | N/A | N/A | N/A | N/A |
| E’gui | Nanning Zoo | 2 | Short | Long | N. siki | + | − | N. siki | N. siki |
| Qingguangyan | Nanning Zoo | 2 | Short | Long | N. siki | N/A | N/A | N/A | N/A |
| Laoer | Nanning Zoo | 2 | Short | Long | N. siki | N/A | N/A | N/A | N/A |
| Da-Shan | Nanning Zoo | 2 | Short | Long | N. siki | + | − | N. gabriellae | N. siki ♂ × (N. siki ♂ × N. gabriellae ♀) ♀ |
| 316 | Nanning Zoo | 1 | Short | Long | N. gabriellae | − | + | N. gabriellae | N. gabriellae |
| Bai-Shou | Nanning Zoo | 1 | Short | Long | N. gabriellae | − | + | N. gabriellae | N. gabriellae |
| Jing-Jing | Nanning Zoo | 1 | Short | Long | N. gabriellae | − | + | N. gabriellae | N. gabriellae |
| Mei-Mei | Nanning Zoo | 2 | 1 short, 1 long |
1 short, 1 Long |
N. leucogenys×N. siki | + | − | N. leucogenys | N. leucogenys×N. siki |
| Xiao-Xiao | Nanning Zoo | 1 and a half |
Short | Long | N. gabriellae×N. siki | + | + | N. leucogenys | N. gabriellae×N. siki |
| A-Huang | Nanning Zoo | 1 and a half |
Short | Long | N. gabriellae×N. siki | N/A | N/A | N/A | N/A |
The karyotype and mitochondrial of Da-Shan suggest different specific affinities. +: The positive result of PCR using primer set BP or HAS. −: The negative result of PCR using primer set BP or HAS. N/A: Not available.