Figure 4.

Long-term treatment with Alda-1 reduces myocyte apoptosis in chronic heart failure post-MI rats. (A) Representative images of cardiomyocyte apoptosis by Terminal deoxynucleotidyl-transferase-mediated dUTP nick-end labeling assay in each group; myocytes were detected with anti-MYH7 antibody (red) and nuclei were counterstained with DAPI (blue). (B) Quantification of apoptosis from (A), expressed as percentage of apoptotic cells out of the total number of cardiac cells; n=4/group. (C) Caspase-3 activity in heart tissue from each group; 3 replicates of each sample, n=4/group. (D) Protein expression levels of cleaved-caspase-3 were determined by western blot analysis; GAPDH was used as the loading control. ^{^^}P<0.001 vs. Sham, **P<0.001 vs. MI. MI, myocardial infarction; MYH7, myosin-7.