Figure 2.

Icariin suppresses the apoptosis of HPDLCs. HPDLCs were administered icariin at different concentrations (0, 10, 20 and 50 µM) for 24 h. (A) Cell apoptosis was determined using immunofluorescence assay (magnification, ×400). (B) Histogram representing the statistical analysis of the rate of cell apoptosis. The experiments were repeated at least 3 times with similar results, and data are presented as the mean ± standard deviation. *P<0.05 and ***P<0.001 vs. HPDLCs. HPDLC, human periodontal ligament fibroblast; PI, propidium iodide.