Figure 4.

Icariin enhances HPDLC osteogenic differentiation ability. HPDLCs were administered icariin at different concentrations (0, 10, 20 and 50 µM) for 24 h. (A) The levels of BMP2, Col I and OPG were detected by western blotting. (B) ELISA was employed to evaluate the ALP levels. The experiments were repeated at least 3 times with similar results, and data are presented as the mean ± standard deviation. *P<0.05 vs. HPDLCs. HPDLC, human periodontal ligament fibroblast; BMP2, bone morphogenetic protein 2; Col I, collagen I; OPG, osteoprotegerin; ALP, alkaline phosphatase.