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. 2018 Jun 29;18(3):2531–2540. doi: 10.3892/mmr.2018.9233

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effect of CUL4A on cell proliferation ability. (A) Evaluation of the effects of CUL4A overexpression and CUL4A knockdown on the cell proliferation of HEPG2 and MHCC97-H liver cancer cells using a Cell Counting Kit-8 assay. (B) Propidium iodide staining followed by flow cytometry was performed to analyze the cell cycle distribution in control, CUL4A overexpression and CUL4A-siRNA HEPG2 and MHCC97-H cells. (C) Percentage of HEPG2 or MHCC97-H cells in each phase. Similar results were obtained in three independent experiments. Results are presented as the mean ± standard error of the mean. *P<0.05 vs. control group. CUL4A, cullin 4A; siRNA, small interfering RNA.