Fig. 5.

The comparison of NPM1 transcript levels estimated from the analysis of RNA-seq data. Boxplots A and B present TPM values. For clear presentation, NPM1 transcripts were divided into two plots according to the level of expression: (a) high and (b) low. Boxplots do not show 8 outliers for NPM1.1, 6 outliers for NPM1.7, and 2 outliers for NPM1.10. A correlation plot (c) shows Spearman correlation coefficients for each pair of NPM1 transcripts, ranked according to the similarity of expression. Color intensity and the size of the circles are proportional to the correlation coefficients. Circles smaller than an empty one under the plot means not statistically significant correlation. d The ratio of NPM1 splice variants in particular samples. Legend is above the plot. e Expression level of SRSF8 gene, encoding one of the splicing factors from the Ser/Arg-rich protein family. Control samples are indicated in pink (BM-HV—bone marrow sample from a healthy volunteer; PB-HV—a pool of 12 peripheral blood samples from healthy volunteers) whereas AML samples in grey (ID014-ID103—AML at the time of first diagnosis, AML-T1—AML sample after therapy), except for one sample (ID074, green) with extremely higher level of SRFS8 expression