Fig. 2.

CD3⁺CD4⁺IL-17⁺, CD4⁺CD25⁺CD127⁺FOXP3⁺ and CD4⁺CD25⁺CD127⁻FOXP3⁺ cells analysis model used in flow cytometry. a Representative scheme of the analysis used to identify CD3⁺CD4⁺IL-17⁺ T cell sub-population through the identification of its cytokine and dot plots illustrate isotype control used to make the quadrants. b Representative scheme of the analysis used to identify Treg cell (CD4⁺CD25⁺CD127⁺FOXP3⁺) and activated Treg cells (CD4⁺CD25⁺CD127⁻FOXP3⁺). c, d Flow cytometry histograms show the isotype controls and cells with positive reactivity with human monoclonal antibodies. Black line shows the isotype control and red line shows cells