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. 2018 Aug 20;19:155. doi: 10.1186/s12931-018-0854-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Mean ± SD (a) C-4-S and (b) C-6-S levels (per unit volume of lung tissue; arbitrary units, AU) in the peri-alveolar region of the lung measured in control fetuses (131d saline-infused, n = 5; 124d antenatal saline-treatment, n = 6; 143d sham operated, n = 6) and fetuses exposed to cortisol-infusion, (131d n = 6), antenatal betamethasone treatment (Beta , 124d, n = 6) or bilaterally adrenalectomised (ADX, 143d, n = 6). Immunolocalisation of C-4-S and C-6-S in the perialveolar region of lungs from (c) saline and cortisol-treated sheep, (d) saline and betamethasone-treated sheep, and (e) sham and ADX-operated sheep. *p < 0.05 compared to controls. Scale = 25μm