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. 2018 Aug 20;15:231. doi: 10.1186/s12974-018-1279-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Deficiency of endogenous TSG-6 leads to an exaggerated proinflammatory microglial phenotype. Immunofluorescence labeling and quantification analysis (a–d) and western blot (e, f) and showing that inhibition of TSG-6 further enhanced the increased levels of CD86 and the enhanced CD163 levels was decreased 24 h after SAH compared with the scramble siRNA group. The samples for western blot are tissue lysates obtained from the left temporal lobe of the brain. White arrows indicate typical cells. Values of the relative densitometric analysis are expressed as mean ± SD, Scale bar = 20 μm, n = 6 in each group. **P < 0.01; ***P < 0.001