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. 2018 Aug 21;15:27. doi: 10.1186/s12014-018-9203-3

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Representative fluorescence images and quantification of FITC-PSA staining. A The detection of spontaneous acrosome reaction in the IVF pregnancy group; B The detection of spontaneous acrosome reaction in the R-ICSI pregnancy group; C The quantification of spontaneous acrosome reaction between the IVF-pregnancy group (n = 20) and the R-ICSI pregnancy group (n = 20). Acrosome-intact sperm is marked by asterisk, and acrosome-reacted sperm is marked by arrow. Each bar indicates 10 μm