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. 2018 Aug 14;5:75. doi: 10.3389/fmolb.2018.00075

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Copyright © 2018 Martinelli, Cuviello, Pace, Armentano, Miglionico, Ostuni and Bisaccia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of Probenecid on some ABC genes and ABCC6 and ABCB1 proteins. (A) Fold change in some ABCs mRNA levels compared to the control cells. Results are represented as the mean ± S.D. (n = 3). **p < 0.01, Student's t-test. (B,C) Representative western blot and densitometric analysis of the ABCC6 and ABCB1 in three independent experiments (means ± SD). ***p < 0.001, Student's t-test. β-actin was used as a loading control. All the experiments were performed treating HepG2 cells with 250 μM of Probenecid for 48 h. DMSO-treated cells were used as control.