FIG 4.

Detection of APN1 expression in individual larvae after dsRNA feeding. (A) Control larvae fed with water. (B) Larvae fed with lysed HT115 bacteria. (C) Larvae fed with lysed HT115 bacteria producing dsRNA-APN1. (D) Larvae fed with lysed HT115 bacteria producing dsRNA-APN1 and treated with Cry1Ca. The midgut tissue was dissected for individual larvae. Seven larvae were dissected per treatment. Lanes 1 to 7 correspond to different individual larvae. Individual midgut tissues were homogenized in PBS, and the supernatant samples recovered after centrifugation were loaded on 10% SDS-PAGE gels. Proteins were electrotransferred to PVDF, and the 170-kDa APN1 protein was detected by the use of anti-APN1 antibody and Western blot assays. The numbers on the left refer to molecular weight standards (in kilodaltons).