TABLE 1.
MIC of BAC and antibiotics for isolates of the same species
| Species | 16S rRNA gene identity (%) | ANIa (%) | MIC (mg/liter)b |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| BAC | TET | CIP | CHL | POL | KAN | RIF | AMP | |||
| Achromobacter denitrificans DP | —c | — | 15.6 | <3.1 | <0.8 | 3.1 | <0.4 | 100 | 12.5 | 200 |
| Achromobacter sp. DPB | 97.8 | 87.5 | 31.3 | 25 | 1.6 | 25 | <0.4 | 25 | 3.1 | <6.3 |
| Achromobacter sp. B | 97.7 | 87.5 | 125 | 200 | 12.5 | 100 | 3.1 | 100 | 12.5 | <6.3 |
| Citrobacter freundii (human)d | — | — | <7.8 | 1.6 | 0.4 | 12.5 | 0.2 | 6.3 | 25 | 800 |
| Citrobacter freundii DP | 98.5 | N/A | 62.5 | 1.6 | 1.6 | 12.5 | 0.8 | 6.3 | 25 | 12.5 |
| Citrobacter freundii DPB | 99.5 | 94.2 | 62.5 | 1.6 | 0.8 | 12.5 | 0.4 | 6.3 | 25 | 25 |
| Klebsiella michiganensis (type strain)e | — | — | 15.6 | 1.6 | <0.1 | 6.3 | 1.6 | 200 | 12.5 | 200 |
| Klebsiella michiganensis DP | 99.1 | N/A | 62.5 | 1.6 | 1.6 | 12.5 | 0.8 | 25 | 12.5 | 100 |
| Klebsiella michiganensis DPB | 99.9 | 99.4 | 62.5 | 1.6 | 1.6 | 12.5 | 1.6 | 25 | 25 | 100 |
| Pseudomonas aeruginosa DP | — | — | 50 | 12.5 | 0.1 | 50 | 0.2 | 100 | 12.5 | 3,200 |
| Pseudomonas aeruginosa DPB | 100 | 100 | 200 | 6.25 | 0.2 | 100 | 0.2 | 100 | 25 | 3,200 |
a
ANI, average nucleotide identity.
b
A range of antibiotic and BAC concentrations (0.4 to 3,200 mg/liter) was tested. TET, tetracycline; CIP, ciprofloxacin; CHL, chloramphenicol; POL, polymyxin B; KAN, kanamycin; RIF, rifampin; AMP, ampicillin.
c
—, control isolate of the same or closely related species for comparisons.
d
C. freundii human strain was 4_7_47_CFAA, accession no. ADLG00000000.1, of the Human Microbiome Project (http://www.hmpdacc.org/).
e
K. michiganensis type strain was KCTC 1686, accession no. CP003218.1, of the Korean Collection for Type Cultures.