FIGURE 2.

Relaxation of uterine arteries. Relaxation of UtAs from WT and eNOS^−/− mice in response to Acetlycholine (Ach) (A) and Sodium Nitroprusside (SNP) (B). Relaxation in response to Ach was significantly reduced, whereas SNP induced relaxation was significantly enhanced in eNOS^−/− mice compared to WT irrespective of treatment. Pomegranate juice supplementation significantly reduced the relaxation responses to both Ach and SNP in WT but not eNOS^−/− animals. Data are mean ± SEM of averages of 2–4 vessels/animal N = 14–20 dams/group. EC₅₀ doses of both Ach (C) and SNP (D) were higher in WT animals supplemented with PJ representing a decreased sensitivity of UTAs following supplementation. n = 22–31 vessels, line = median. ^∗P < O.05, ^∗∗∗P < 0.001, significant effect of genotype. ^ϕP < 0.05, significant effect of treatment.