Fig. 2. Absorption and emission spectra of (a) mAb–CSPP and (b) mAb–Cy3 conjugates. Conjugate concentration: 0.2 mg mL^–1. (c) SDS-PAGE of monoclonal antibody cetuximab (mAb), and mAb–CSPP and mAb–Cy3 conjugates. Left: the protein marker was excited by the Cy5 (635 nm) and Cy3 (532 nm) channels, mAb–CSPP was excited by UV channel and the mAb–Cy3 was excited at Cy3 channel. Right: All protein bands were stained with Coomassie Brilliant Blue showing the intact antibody and the partly reduced antibody with two heavy chains left.