Fig. 3. Bright-field and fluorescent images of (a–d) HCC827 cancer cells and (e–h) COS-7 normal cells incubated with 10 μg mL–1 of mAb–CSPP and mAb–Cy3 conjugates for 12 h. Images were taken (a, b, e and f) in the probe medium without washing and (c, d, g and h) in the medium without probe after washing. Conditions: for mAb–Cy3, λex = 510–550 nm, dichroic mirror = 570 nm; for mAb–CSPP, λex = 400–440 nm, dichroic mirror = 455 nm. Exposure time = 1 s; scale bar = 30 μm.
