Fig. 4. (a) LSCM images of HCC827 cells incubated with 10 μg mL–1 mAb–CSPP conjugates at different incubation times without PBS washing and 10 μg mL–1 mAb–Cy3 at different incubation times after PBS washing. Conditions: for mAb–Cy3, λex = 560 nm, emission filter = 563–700 nm. For mAb–CSPP, λex = 405 nm, emission filter = 550–700 nm. Scale bar: 20 μm. (b) Flow cytometric analysis of HCC827 cells after incubation with mAb–CSPP and mAb–Cy3 conjugates at different time intervals. The plot shows the relative fluorescence intensity (MFI/MFI1 h) with probe incubation time, where MFI1 h is the mean fluorescence intensity after probe incubation for 1 h.
