Fig. 5. (a) LSCM images of HCC827 cells stained with 10 μg mL–1 mAb–CSPP or mAb–Cy3 conjugates for 4 h and then co-stained with LysoTracker Green (LTG) for 5 min. (b) LSCM images of HCC827 cells stained with 10 μg mL–1 mAb–CSPP for 24 h, followed by staining with LTG/Mito-Tracker Orange (MTO). Conditions: for mAb–Cy3, λex = 560 nm, emission filter = 563–700 nm. For mAb–CSPP, λex = 405 nm, emission filter = 550–700 nm. For LTR, λex = 488 nm, emission filter = 495–535 nm. For MTO, λex = 560 nm, emission filter = 565–585 nm. Scale bar: 20 μm.
