Table 1. MRMHR channel parameters used for quantification of the lipid mediators via LC-MS/MS.
| Common Name | IS | DP (V) | CE (±2V) | Precursor m/z | Product m/z |
|---|---|---|---|---|---|
| *Window of 0.05 Da | |||||
| LTC4 | LTC4-d5 | -23 | -40 | 624.2969 | 272.0893 |
| LTD4 | LTD4-d5 | -30 | -29 | 495.2531 | 143.0464 |
| LTE4 | LTE4-d5 | -30 | -30 | 438.2312 | 235.1529 |
| RvD2 | RvD1-d4 | -23 | -29 | 375.2170 | 141.0538 |
| 6-keto-PGF1α | 6-keto-PGF1α-d4 | -26 | -24 | 369.2274 | 163.1132 |
| PGG2 | PGE2-d4 | -27 | -28 | 367.2121 | 235.0903 |
| 10(S),17(S)-diHDoHE | RvD1-d4 | -23 | -26 | 359.2222 | 153.0920 |
| PGF2α | PGF2α-d4 | -28 | -38 | 353.2326 | 309.2179 |
| PGE2 | PGE2-d4 | -27 | -25 | 351.2168 | 189.1294 |
| PGD2 | PGD2-d4 | -31 | -25 | 351.2168 | 189.1295 |
| 15-keto-PGE2 | PGE2-d4 | -23 | -28 | 349.2017 | 287.1995 |
| 11,12-diHETrE | 5-HETE-d8 | -37 | -29 | 337.2377 | 167.1086 |
| LTB4 | 12-epi-LTB4-d4 | -25 | -28 | 335.2221 | 129.0552 |
| PGJ2 | PGD2-d4 | -23 | -23 | 333.2065 | 189.1290 |
| 11-HETE | 12-HETE-d8 | -25 | -29 | 319.2272 | 167.1078 |
| 5-oxo-ETE | 5-oxo-ETE-d7 | -23 | -33 | 317.2116 | 203.1825 |
| 15-deoxi-δ12,14-PGJ2 | 15-deoxi-δ-12,14-PGJ2-d4 | -24 | -31 | 315.1962 | 203.1422 |
| AA | AA-d8 | -22 | -31 | 303.2324 | 259.2447 |
| EPA | AA-d8 | -32 | -30 | 301.2172 | 257.2306 |
| 11-trans-LTD4 | LTD4-d5 | -30 | -30 | 495.2620 | 143.0468 |
| TXB2 | TXB2-d4 | -22 | -24 | 369.2277 | 169.0874 |
| 20-OH-PGE2 | PGE2-d4 | -30 | -31 | 367.2119 | 287.1995 |
| 7(R)-Maresin-1 | RvD1-d4 | -30 | -27 | 359.2221 | 250.1228 |
| 19-OH-PGB2 | PGE2-d4 | -28 | -39 | 349.2015 | 175.1131 |
| 14,15-diHETrE | 5-HETE-d8 | -22 | -29 | 337.2377 | 207.1393 |
| 5,6-diHETE | 5-HETE-d8 | -35 | -25 | 335.2211 | 115.0403 |
| PGB2 | PGE2-d4 | -27 | -32 | 333.2067 | 175.1125 |
| 5-HETE | 5-HETE-d8 | -22 | -31 | 319.2275 | 115.0388 |
| 12-oxo-ETE | 5-oxo-ETE-d7 | -35 | -30 | 317.2116 | 153.1300 |
| 20-OH-LTB4 | 12-epi-LTB4-d4 | -27 | -30 | 351.2195 | 129.0544 |
| 6-trans-LTB4 | 12-epi-LTB4-d4 | -20 | -27 | 335.2248 | 129.0557 |
| 12-HETE | 12-HETE-d8 | -27 | -30 | 319.2289 | 179.1071 |
| 15-oxo-ETE | 5-oxo-ETE-d7 | -15 | -28 | 317.2145 | 113.0979 |
| 5S,6R-LXA4 | 5S,6R-LXA4-d5 | -22 | -25 | 351.2192 | 217.1598 |
| 8-HETE | 5-HETE-d8 | -22 | -30 | 319.2297 | 155.0720 |
| PGH2 | PGE2-d4 | -29 | -24 | 351.2186 | 271.2051 |
| 12-HETE | 12-HETE-d8 | -27 | -30 | 319.2291 | 179.1081 |
| 20-HETE | 15-HETE-d8 | -18 | -34 | 319.2293 | 289.2165 |
| 15-HETE | 15-HETE-d8 | -33 | -30 | 319.2351 | 175.1496 |
| 5,6-DiHETrE | 5-HETE-d8 | -27 | -31 | 337.2427 | 145.0513 |
| 6-keto-PGF1α-d4 | - | -35 | -30 | 373.2534 | 211.1287 |
| TXB2-d4 | - | -24 | -19 | 373.2524 | 173.1132 |
| PGE2-d4 | - | -37 | -23 | 355.2424 | 275.2333 |
| PGD2-d4 | - | -46 | -18 | 355.2424 | 237.1451 |
| 15-deoxi-δ-12,14-PGJ2-d4 | - | -27 | -23 | 319.2216 | 275.2341 |
| PGF2α-d4 | - | -37 | -39 | 357.2583 | 177.1486 |
| LTB4-d4 | - | -29 | -43 | 339.2479 | 183.0386 |
| 5-HETE-d8 | - | -24 | -25 | 327.2791 | 116.0473 |
| 12-HETE-d8 | - | -34 | -25 | 327.2791 | 184.1390 |
| 15-HETE-d8 | - | -47 | -27 | 327.2791 | 226.1840 |
| 5-oxo-ETE-d7 | - | -29 | -32 | 324.2554 | 210.2230 |
| RvD1-d4 | - | -24 | -27 | 350.2491 | 220.1764 |
| LTC4-d5 | - | -20 | -39 | 629.3274 | 272.0899 |
| LTE4-d5 | - | -34 | -27 | 443.2634 | 338.2218 |
| AA-d8 | - | -35 | -36 | 311.2832 | 237.0907 |
| LTD4-d5 | - | -26 | -28 | 500.2848 | 482.2772 |
| 12-epi-LTB4-d4 | - | -36 | -26 | 339.2471 | 197.1152 |
| 5S,6R-LXA4-d5 | - | -30 | -28 | 356.2491 | 222.1907 |