Table 2. Retention time, r2, and LLOQ for all lipid mediator targets analyzed at pH 5.8.
| Common name | Retention time (min) | r2 | LLOQa(pg) |
|---|---|---|---|
| * Calibration curves include six runs, with ten concentrations. | |||
| 20-OH-LTB4 | 3.06 | 0.9986 | 0.05 |
| LTC4 | 5.09 | 0.9987 | 0.09 |
| PGB2 | 8.70 | 0.9993 | 0.02 |
| 15-keto-PGE2 | 5.99 | 0.9994 | 0.05 |
| 20-OH-PGE2 | 1.80 | 0.9989 | 0.05 |
| TXB2 | 4.06 | 0.9987 | 0.05 |
| LXA4 | 5.93 | 0.9993 | 0.09 |
| PGD2 | 5.56 | 0.9988 | 0.05 |
| 6-keto-PGF1α | 2.45 | 0.9963 | 0.18 |
| PGE2 | 5.20 | 0.9993 | 0.02 |
| RvD2 | 6.23 | 0.9989 | 0.09 |
| PGF2α | 4.63 | 0.9968 | 0.09 |
| 19-OH-PGB2 | 2.28 | 0.9982 | 0.09 |
| PGG2 | 6.73 | 0.9919 | 0.37 |
| LTB4 | 10.54 | 0.9981 | 0.02 |
| LTD4 | 6.90 | 0.9993 | 0.05 |
| LTE4 | 7.90 | 0.9987 | 0.09 |
| 6-trans-LTB4 | 10.09 | 0.9977 | 0.05 |
| 11-trans-LTD4 | 6.90 | 0.9991 | 0.09 |
| PDx | 10.40 | 0.9991 | 0.05 |
| MaR-1 | 10.21 | 0.9986 | 0.05 |
| PGH2 | 7.61 | 0.9966 | 0.09 |
| PGJ2 | 8.65 | 0.9993 | 0.05 |
| 15-deoxy-δ-12,14-PGJ2 | 13.23 | 0.9970 | 0.09 |
| 5-HETE | 14.41 | 0.9989 | 0.05 |
| AA | 17.17 | 0.9675 | 0.09 |
| 5-oxo-ETE | 14.68 | 0.9986 | 0.02 |
| 20-HETE | 13.35 | 0.9977 | 0.09 |
| 5,6-DiHETE | 12.91 | 0.9992 | 0.02 |
| 12-HETE | 14.22 | 0.9993 | 0.05 |
| 8-HETE | 14.22 | 0.9984 | 0.02 |
| 11-HETE | 14.08 | 0.9972 | 0.05 |
| 12-oxo-ETE | 14.69 | 0.9980 | 0.05 |
| 15-oxo-ETE | 13.99 | 0.9969 | 0.05 |
| 11,12-DiHETrE | 12.94 | 0.9981 | 0.05 |
| 14,15-DiHETrE | 12.73 | 0.9931 | 0.05 |
| EPA | 16.71 | 0.9951 | 0.09 |
| 5,6-DiHETrE | 13.22 | 0.9974 | 0.09 |
| 15-HETE | 13.89 | 0.9967 | 0.09 |
aLower limit of quantification values were considered for accuracy within 20% of the nominal concentration (CV ≤20%).