Table 3. Quality control analysis of lipid mediators to evaluate the precision and accuracy of the MRMHR method.
| Analytes |
Intraday (n=5)
a |
Interday (3 days)
b |
|||
|---|---|---|---|---|---|
| RSD % c | RE % d | RSD % c | RE % d | ||
| 20-OH-LTB4 | LQC | 16.44 | 11.39 | 11.08 | 4.44 |
| MQC | 5.98 | 6.90 | 12.90 | 8.67 | |
| HQC | 4.07 | 3.36 | 7.05 | 1.04 | |
| LTC4 | LQC | 10.49 | 7.78 | -- | -- |
| MQC | 5.62 | 7.67 | 14.37 | 5.07 | |
| HQC | 4.38 | 0.77 | 14.48 | 3.66 | |
| PGB2 | LQC | 5.93 | 0.56 | 12.73 | 19.44 |
| MQC | 3.47 | 1.33 | 8.72 | 4.53 | |
| HQC | 5.31 | 4.01 | 7.60 | 2.98 | |
| 15-keto-PGE2 | LQC | 11.38 | 1.39 | 11.03 | 1.11 |
| MQC | 5.24 | 0.37 | 9.12 | 4.20 | |
| HQC | 4.84 | 2.61 | 5.06 | 2.48 | |
| 20-OH-PGE2 | LQC | 9.95 | 2.50 | 19.11 | 3.33 |
| MQC | 5.62 | 6.27 | 9.69 | 0.47 | |
| HQC | 5.31 | 3.31 | 7.88 | 4.86 | |
| TXB2 | LQC | 8.32 | 4.72 | 11.26 | 2.78 |
| MQC | 7.53 | 4.57 | 8.40 | 4.13 | |
| HQC | 5.36 | 4.29 | 5.04 | 3.76 | |
| LXA4 | LQC | 17.74 | 6.39 | 11.28 | 8.89 |
| MQC | 7.93 | 2.17 | 7.47 | 1.47 | |
| HQC | 1.63 | 0.97 | 4.26 | 0.20 | |
| PGD2 | LQC | 6.58 | 0.56 | 10.53 | 4.44 |
| MQC | 4.11 | 3.27 | 4.09 | 3.73 | |
| HQC | 4.16 | 0.84 | 4.73 | 3.78 | |
| 6-keto-PGF1α | LQC | 11.07 | 5.00 | 19.10 | 15.00 |
| MQC | 10.59 | 10.10 | 7.89 | 2.27 | |
| HQC | 8.46 | 10.14 | 8.88 | 5.90 | |
| PGE2 | LQC | 5.99 | 9.28 | 5.95 | 0.01 |
| MQC | 1.25 | 3.43 | 1.63 | 0.93 | |
| HQC | 3.76 | 2.13 | 7.35 | 1.08 | |
| RvD2 | LQC | 10.64 | 5.83 | 6.56 | 3.33 |
| MQC | 9.69 | 0.77 | 7.19 | 0.77 | |
| HQC | 2.55 | 5.27 | 6.97 | 8.96 | |
| PGF2α | LQC | 8.35 | 12.78 | -- | -- |
| MQC | 9.65 | 4.63 | 15.00 | 4.31 | |
| HQC | 2.33 | 0.43 | 2.99 | 1.38 | |
| 19-OH-PGB2 | LQC | 8.80 | 11.11 | 12.71 | 13.33 |
| MQC | 7.06 | 4.73 | 12.13 | 10.60 | |
| HQC | 7.14 | 8.72 | 8.30 | 2.40 | |
| PGG2 | LQC | -- | -- | -- | -- |
| MQC | 8.88 | 4.00 | -- | -- | |
| HQC | 13.70 | 2.93 | -- | -- | |
| LTB4 | LQC | 10.83 | 10.00 | 14.76 | 6.11 |
| MQC | 5.23 | 1.77 | 8.43 | 6.53 | |
| HQC | 2.96 | 4.70 | 2.86 | 1.94 | |
| LTD4 | LQC | 12.34 | 1.39 | -- | -- |
| MQC | 5.20 | 0.83 | 8.03 | 12.53 | |
| HQC | 2.73 | 0.57 | 5.06 | 6.34 | |
| LTE4 | LQC | 8.88 | 3.33 | -- | -- |
| MQC | 7.54 | 0.93 | 9.68 | 5.27 | |
| HQC | 7.70 | 7.28 | 5.08 | 0.18 | |
| 6-trans-LTB4 | LQC | 3.16 | 7.22 | -- | -- |
| MQC | 7.46 | 6.93 | 3.90 | 0.60 | |
| HQC | 5.13 | 2.21 | 7.54 | 3.80 | |
| 11-trans-LTD4 | LQC | 7.96 | 0.01 | 8.86 | 10.56 |
| MQC | 2.77 | 0.50 | 7.43 | 0.40 | |
| HQC | 2.93 | 2.95 | 8.54 | 5.52 | |
| PDx | LQC | 5.17 | 3.89 | 7.04 | 1.11 |
| MQC | 5.57 | 1.93 | 3.66 | 4.00 | |
| HQC | 4.54 | 1.75 | 4.37 | 1.32 | |
| MaR-1 | LQC | 8.79 | 1.67 | -- | -- |
| MQC | 7.27 | 0.80 | 10.83 | 4.80 | |
| HQC | 5.13 | 3.00 | 8.58 | 0.32 | |
| PGH2 | LQC | 14.41 | 7.50 | -- | -- |
| MQC | 7.91 | 6.30 | 13.56 | 6.33 | |
| HQC | 5.17 | 0.27 | 7.87 | 3.78 | |
| PGJ2 | LQC | 6.28 | 8.06 | 13.74 | 9.44 |
| MQC | 5.02 | 4.47 | 9.16 | 9.27 | |
| HQC | 3.51 | 0.40 | 4.34 | 2.34 | |
| 15-deoxy-δ-12,14-PGJ2 | LQC | 6.00 | 7.78 | -- | -- |
| MQC | 4.78 | 3.40 | 3.27 | 2.53 | |
| HQC | 3.80 | 5.95 | 3.73 | 4.98 | |
| 5-HETE | LQC | 9.68 | 5.28 | 8.13 | 7.78 |
| MQC | 4.24 | 4.70 | 3.33 | 8.87 | |
| HQC | 4.45 | 2.28 | 2.92 | 6.38 | |
| AA | LQC | 8.02 | 5.00 | -- | -- |
| MQC | 14.82 | 0.70 | -- | -- | |
| HQC | -- | -- | 14.95 | 0.22 | |
| 5-oxo-ETE | LQC | 6.15 | 1.94 | -- | -- |
| MQC | 2.47 | 1.80 | 12.98 | 11.27 | |
| HQC | 3.29 | 1.23 | 2.07 | 6.30 | |
| 20-HETE | LQC | 2.72 | 9.17 | -- | -- |
| MQC | 5.04 | 0.53 | 5.82 | 1.87 | |
| HQC | 4.19 | 2.53 | 9.49 | 5.82 | |
| 5,6-DiHETE | LQC | 6.46 | 1.94 | 12.68 | 16.67 |
| MQC | 2.51 | 6.40 | 4.51 | 3.13 | |
| HQC | 3.32 | 1.02 | 2.87 | 0.98 | |
| 12-HETE | LQC | 4.43 | 2.22 | 11.41 | 3.33 |
| MQC | 4.93 | 4.13 | 6.05 | 6.87 | |
| HQC | 3.85 | 4.83 | 3.26 | 1.28 | |
| 8-HETE | LQC | 4.50 | 1.39 | 14.26 | 1.67 |
| MQC | 6.17 | 4.13 | 4.67 | 2.27 | |
| HQC | 3.87 | 1.00 | 3.63 | 2.74 | |
| 11-HETE | LQC | 4.68 | 9.17 | 8.61 | 10.00 |
| MQC | 1.27 | 5.60 | 3.51 | 1.13 | |
| HQC | 6.05 | 8.69 | 2.69 | 0.36 | |
| 12-oxo-ETE | LQC | 7.45 | 2.50 | 17.21 | 8.89 |
| MQC | 4.12 | 5.33 | 4.71 | 1.40 | |
| HQC | 3.93 | 3.94 | 9.52 | 1.52 | |
| 15-oxo-ETE | LQC | 2.35 | 7.78 | 9.31 | 1.67 |
| MQC | 3.43 | 6.20 | 5.44 | 1.47 | |
| HQC | 2.83 | 6.33 | 4.87 | 1.74 | |
| 11,12-DiHETrE | LQC | 4.84 | 0.56 | 9.19 | 11.11 |
| MQC | 2.09 | 8.87 | 2.41 | 2.40 | |
| HQC | 3.64 | 6.11 | 1.92 | 0.60 | |
| 14,15-DiHETrE | LQC | 3.09 | 0.83 | 12.46 | 12.78 |
| MQC | 6.94 | 7.07 | 7.82 | 13.73 | |
| HQC | 7.28 | 9.71 | 4.66 | 3.60 | |
| EPA | LQC | 8.52 | 5.00 | -- | -- |
| MQC | 8.60 | 0.67 | 11.25 | 12.80 | |
| HQC | 4.33 | 5.25 | 5.20 | 2.54 | |
| 5,6-DiHETrE | LQC | 3.13 | 5.28 | 15.91 | 0.28 |
| MQC | 2.37 | 4.07 | 3.26 | 4.80 | |
| HQC | 2.02 | 2.29 | 2.46 | 3.06 | |
| 15-HETE | LQC | 8.75 | 0.28 | 12.91 | 8.89 |
| MQC | 9.10 | 3.13 | 8.07 | 1.30 | |
| HQC | 5.44 | 8.51 | 5.30 | 0.52 | |
aNumber of measurements
bnumber of days
crelative standard deviation, in percentage points (%)
drelative error in percentage points (%).