Table 4. Mean recovery and matrix effect of the lipid mediator targets using SPE extraction in HQC, MQC, and LQC levels of IS in plasma samples.
| Internal Standard |
Recovery Rate (%±SD)a |
Matrix Effect (%± SD)b |
||||
|---|---|---|---|---|---|---|
| HQCc | MQCd | LQCe | HQCc | MQCd | LQCe | |
| *n=6, injected in triplicate. | ||||||
| 15-HETE-d8 | 80.5±12.1 | 99.6±11.7 | 135.2±25.4 | 106.4±7.6 | 118.8±20.0 | 139.7±11.6 |
| TXB2-d5 | 59.9±7.9 | 70.4±6.8 | 75.3±11.1 | 70.9±3.9 | 72.1±9.18 | 70.4±7.1 |
| 6-keto-PGF1_-d4 | 136.2±15.1 | 145.8±25.3 | ------ | 145.3±14.9 | 154.4±27.4 | ----- |
| PGE2-d4 | 74.7±8.6 | 91.7±5.6 | 94.6±13.2 | 88.5±6.4 | 100.1±15.3 | 93.7±10.4 |
| PGF2_-d4 | 84.4±8.2 | 95.6±4.2 | 89.0±29.3 | 101.9±9.7 | 106.7±23.7 | 96.3±17.8 |
| LXA4-d5 | 74.1±7.5 | 84.1±10.9 | ----- | 101.8±9.2 | 96.8±18.8 | 150.1±24.4 |
| RvD1-d5 | 76.1±9.1 | 92.0±9.4 | 115.1±13.7 | 100.6±10.2 | 107.2±21.4 | 107.0±21.9 |
| PGD2-d4 | 44.8±3.6 | 48.0±8.2 | 57.6±12.7 | 41.3±3.4 | 48.5±8.5 | 59.5±13.7 |
| 12-epi-LTB4-d4 | 79.7±3.4 | 98.0±11.2 | 104.2±14.4 | 97.1±6.8 | 109.7±17.4 | 116.3±11.9 |
| LTE4-d5 | 62.2±7.5 | 69.9±7.9 | ----- | 132.1±6.6 | 141.5±24.9 | 201.8±102.8 |
| AA-d8 | 51.3±1.9 | 27.9±4.8 | 21.9±5.1 | 58.7±5.9 | 27.6±6.7 | 26.1±6.4 |
| 15-deoxy-δ-12,14-PGJ2-d4 | 69.4±9.5 | 94.0±22.9 | 191.8±29.0 | 109.8±8.2 | 131.1±32.9 | 167.7±30.1 |
| 5-HETE-d8 | 76.8±10.4 | 96.7±8.7 | 96.6±13.4 | 99.1±6.5 | 102.2±15.4 | 117.7±7.4 |
| 5-oxo-ETE-d7 | 81.9±12.9 | 87.9±12.2 | 108.4±25.5 | 99.8±6.8 | 93.5±16.7 | 120.7±24.9 |
| 12-HETE-d8 | 78.7±11.9 | 99.2±8.0 | 126.1±14.8 | 104.2±8.5 | 121.7±21.2 | 138.7±14.0 |
aRecovery were performed by comparing the extracted samples with unextracted standards that represent 100%.
bMatrix effect expressed as the ratio of the mean peak area of an analyte spiked post-extraction to the mean peak area of the same analyte standards multiplied by 100. A value of >100% indicates ionization enhancement, and a value of <100% indicates ionization suppression.
cHigh-concentration Quality Control.
dMedium-concentration Quality Control.
eLow-concentration Quality Control.