Table 6. Mean recovery and matrix effect of the lipid mediator targets using SPE extraction in HQC, MQC, and LQC levels of IS in cell culture medium.

Internal Standard	Recovery Rate (%±SD)a			Matrix Effect (%± SD)b
	HQCc	MQCd	LQCe	HQCc	MQCd	LQCe
*n=6, injected in triplicate.
15-HETE-d8	42.7±5.8	56.6±12.8	52.3±8.9	79.9±12.9	114.1±3.6	118.9±9.8
TXB2-d5	58.3±6.4	58.9±2.9	58.6±5.2	54.2±6.9	74.59±4.9	62.0±10.1
6-keto-PGF1α-d4	34.4±4.5	74.9±17.1	-------	95.5±7.7	126.9±33.3	-------
PGE2-d4	56.7±3.2	78.8±5.0	71.5±4.9	64.2±12.3	94.3±3.8	80.1±10.2
PGF2_-d4	73.1±7.1	82.5±8.0	74.2±19.2	78.5±17.1	104.4±11.6	79.9±20.9
LXA4-d5	44.5±5.7	51.5±4.3	-------	48.6±6.6	62.4±4.9	-------
RvD1-d5	49.9±10.1	51.9±3.7	57.6±7.1	49.2±9.4	67.4 ±5.6	63.4±13.4
PGD2-d4	49.3±8.5	54.8±3.9	56.7±10.8	47.3±8.7	69.4±2.9	73.6±9.3
12-epi-LTB4-d4	61.1±8.2	59.8±3.2	63.0±14.4	66.2±11.9	87.9±5.4	78.4±10.1
LTE4-d5	73.3±36.6	34.3±5.1	80.8±28.1	68.5±15.7	93.6±8.5	114.4±43.2
AA-d8	44.3±8.4	12.3±7.1	-------	47.2±5.3	35.4±3.5	38.7±6.5
15-deoxy-δ-12,14-PGJ2-d4	40.2±5.3	43.8±5.0	59.6±5.4	54.0±9.5	77.3±5.6	83.3±5.3
5-HETE-d8	37.1±4.2	46.3±12.1	35.6±13.5	79.91± 14.3	103.9±5.6	94.9±8.3
5-oxo-ETE-d7	41.5±9.2	36.3±13.2	-------	83.3±16.0	96.4±10.2	81.2±14.3
12-HETE-d8	43.5±6.8	42.4±9.6	42.3±15.2	71.5±12.5	98.9±4.6	109.5±9.8

^aRecovery were performed by comparing the extracted samples with unextracted standards that represent 100%.

^bMatrix effect expressed as the ratio of the mean peak area of an analyte spiked post-extraction to the mean peak area of the same analyte standards multiplied by 100. A value of >100% indicates ionization enhancement, and a value of < 100% indicates ionization suppression.

^cHigh-concentration Quality Control.

^dMedium-concentration Quality Control.

^eLow-concentration Quality Control.