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. Author manuscript; available in PMC: 2018 Aug 21.
Published in final edited form as: Nat Genet. 2013 Sep 1;45(10):1190–1197. doi: 10.1038/ng.2743

Figure 3.

Figure 3

Relative competitive reductions in fitness for isogenic embB, Rv3806c and/or Rv3792 mutants and strain 4C31-16C1-24C1. Each test strain was mixed at approximately a 1:1 ratio with the parental 210 strain and then cultured in the absence of ethambutol to stationary phase. Confirmed initial (inoculation) and final CFUs were used to calculate the relative fitness of each strain compared to strain 210. The means and s.d. of three independent experiments are shown. The results show a fitness cost for the embB306 mutation but not for the other mutations introduced by allelic exchange. The multiple-step mutant strain 4C31-16C1-24C1 also has reduced fitness.