. Author manuscript; available in PMC: 2018 Aug 21.

Published in final edited form as: Nat Genet. 2013 Sep 1;45(10):1190–1197. doi: 10.1038/ng.2743

Table 3.

Ethambutol MICs of isogenic and overexpression strains

Strain	Inserted mutation					Ethambutol MIC (µg/ml)^c

	embB^a	Rv3806c^a	Rv3792^a	nuoD^a	pMV261-Rv3806c^b
210	WT	WT	WT	WT	NA	2
210::Rv3806c-188	WT	p.Val188Ala	WT	WT	NA	4
210::Rv3806c-237	WT	p.Ala237Val	WT	WT	NA	4
210::Rv3806c-240	WT	p.Arg240Cys	WT	WT	NA	4
210::Rv3806c-249	WT	p.Ala249Gly	WT	WT	NA	4
210::Rv3792-198	WT	WT	p.Leu198Leu	WT	NA	8
210::Rv3806c-237/Rv3792-198	WT	p.Ala237Val	p.Leu198Leu	WT	NA	8
210::nuoD-287	WT	WT	WT	p.Ala287Ser	NA	2
210::Rv3806c-237-WT	WT	WT	WT	WT	NA	2
210::Rv3806c-249-WT	WT	WT	WT	WT	NA	2
210::Rv3792-198-WT	WT	WT	WT	WT	NA	2
210::Rv3806c-237/Rv3792-198-WT	WT	p.Ala237Val	WT	WT	NA	4
NJT210GTG	p.Met306Val	WT	WT	WT	NA	16
NJT210GTG::Rv3806c-237	p.Met306Val	p.Ala237Val	WT	WT	NA	32
NJT210GTG::Rv3806c-240	p.Met306Val	p.Arg240Cys p.Gly269Gly	WT	WT	NA	32
NJT210GTG::Rv3806c-249	p.Met306Val	p.Ala249Gly	WT	WT	NA	32
NJT210GTG::Rv3792-198	p.Met306Val	WT	p.Leu198Leu	WT	NA	16
NJT210GTG::Rv3806c-237/Rv3792-198	p.Met306Val	p.Ala237Val	p.Leu198Leu	WT	NA	32
NJT210GTG::nuoD-287	p.Met306Val	WT	WT	p.Ala287Ser	NA	16
NJT210GTG::embB-306-WT	WT	WT	WT	WT	NA	2
210::pMV261	WT	WT	WT	WT	NA	2
210::pMV261-Rv3806c-WT	WT	WT	WT	WT	WT	4
210::pMV261-Rv3806c-188	WT	WT	WT	WT	p.Val188Ala	4
210::pMV261-Rv3806c-237	WT	WT	WT	WT	p.Ala237Val	4
210::pMV261-Rv3806c-240	WT	WT	WT	WT	p.Arg240Cys	4
210::pMV261-Rv3806c-249	WT	WT	WT	WT	p.Ala249Gly	4
NJT210GTG::pMV261	p.Met306Val	WT	WT	WT	NA	16
NJT210GTG::pMV261-Rv3806c-WT	p.Met306Val	WT	WT	WT	WT	32
NJT210GTG::pMV261-Rv3806c-188	p.Met306Val	WT	WT	WT	p.Val188Ala	32
NJT210GTG::pMV261-Rv3806c-237	p.Met306Val	WT	WT	WT	p.Ala237Val	32
NJT210GTG::pMV261-Rv3806c-240	p.Met306Val	WT	WT	WT	p.Arg240Cys	32
NJT210GTG::pMV261-Rv3806c-249	p.Met306Val	WT	WT	WT	p.Ala249Gly	32

WT, wild type, no mutation inserted; NA, not applicable.

Protein alterations encoded by point mutations inserted in the chromosomal genes.

Overexpression of different Rv3806c alleles.

Ethambutol MIC determined by BACTEC 460TB and 7H10 agar proportion. MIC measurements were performed in triplicate by each method; all six replicates produced identical values (as shown) for each strain. MICs for isoniazid (0.05 µg/ml) and/or rifampin (0.05 µg/ml) were identical for all strains listed.