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. 2018 Aug;59(10):4228–4237. doi: 10.1167/iovs.18-24672

Figure 1.

Figure 1

TSLP expression and secretion in primary human corneal epithelial cells in response to P. aeruginosa challenge. HCECs (p4) grown overnight in KBM were treated with heat-killed P. aeruginosa (MOI = 50) for different times. Cell lysates as well as culture media were collected and subjected to PCR and Western blotting analyses. (A) TSLP expression in cultured HCECs challenged with heat-killed P. aeruginosa for 1, 2, 4, and 8 hours was analyzed by real-time PCR. The results are representative of two independent experiments, each with three corneas. *P < 0.05. **P < 0.01 (1-way ANOVA, with Bonferroni Correction). (B) The steady-state expression of TSLP in HCEC cell lysates at indicated times were analyzed by Western blot. (C) TSLP secretion was analyzed by Western blotting of the concentrated culture media of HCECs challenged with heat-killed P. aeruginosa. The data shown are representative of two independent experiments.