Figure 2.

TSLP and TSLPR expression in P. aeruginosa–infected mouse corneal epithelial cells. The centers of B6 mouse corneas were gently scratched with 26-gauge needles (1 mm long; 3 lines) and incubated with 1.0 × 10⁴ CFU of P. aeruginosa. At the indicated times, mouse CECs were collected and subjected to (A) RT-PCR, (B, C) Real-time PCR for TSLP and TSLPR expression in naïve and P. aeruginosa infected mouse CECs, respectively. (D) TSLP expression in mouse CECs was assessed using ELISA. Error bars show standard deviations. The results are representative of two independent experiments, each with 3 corneas. *P < 0.05. **P < 0.01 (1-way ANOVA, with Bonferroni correction).