Figure 4.

Effects of TSLPR neutralization mediated downregulation on the severity of P. aeruginosa keratitis. Mouse corneas were subconjunctivally injected 5 μL control IgG or anti-TSLP at −4 hours. At 0 hours, the corneas were inoculated with 1.0 × 10⁴ CFU of P. aeruginosa. (A) Micrographs and clinical scores of the control (IgG) and TSLPR neutralizing antibody treated corneas at 1, 2, and 3 dpi. Note the increased opacification in anti-TSLPR–treated corneas (right panels). The infected corneas were excised, homogenized, and subjected to (B) bacteria counting, (C) MPO determination (units/cornea), and (D) ELISA determination for IL1β. The results are representative for two independent experiments. **P < 0.01, (t-test).